New evidences on Tau–DNA interactions and relevance to neurodegeneration.

Padmaraju, Vasudevaraju and Shantinath, Satappa Indi and Jagannatha Rao, Kosagi Sharaf (2010) New evidences on Tau–DNA interactions and relevance to neurodegeneration. Neurochemistry International, 57 (1). pp. 51-57.

PDF Neurochemistry International 57 (2010) 51–57.pdf - Published Version Restricted to Registered users only Download (515kB)

Abstract

Tau is mainly distributed in cytoplasm and also found to be localized in the nucleus. There is limited data on DNA binding potential of Tau. We provide novel evidence on nicking of DNA by Tau. Tau nicks the supercoiled DNA leading to open circular and linear forms. The metal ion magnesium (a co-factor for endonuclease) enhanced the Tau DNA nicking ability, while an endonuclease specific inhibitor, aurinetricarboxylic acid (ATA) inhibited the Tau DNA nicking ability. Further, we also evidenced that Tau induces B–C–A mixed conformational transition in DNA and also changes DNA stability. Tau–scDNA complex is more sensitive to DNAse I digestion indicating stability changes in DNA caused by Tau. These findings indicate that Tau alters DNA helicity and integrity and also nicks the DNA. The relevance of these novel intriguing findings regarding the role Tau in neuronal dysfunction is discussed.

Item Type:	Article
Uncontrolled Keywords:	Tau; DNA conformation; DNA nicking; Aurinetricarboxylic acid; Neurodegeneration; Alzheimer's disease
Subjects:	500 Natural Sciences and Mathematics > 07 Life Sciences > 03 Biochemistry & Molecular Biology
Divisions:	Dept. of Biochemistry
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	05 Aug 2011 07:08
Last Modified:	03 Jun 2019 11:34
URI:	http://ir.cftri.res.in/id/eprint/10399

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