Differential partitioning of betacyanins and betaxanthins employing aqueous two phase extraction.

Chandrashekar, J. and Sonika, G. and Madhusudhan, M. C. and Raghavarao, K. S. M. S. (2015) Differential partitioning of betacyanins and betaxanthins employing aqueous two phase extraction. Journal of Food Engineering, 144. pp. 156-163.

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Abstract

Yellow betaxanthins and red purple betacyanins are genetically and structurally closely related pigments, hence making their separation from each other difficult and tedious. Fractionation of betalains into betacyanin and betaxanthin could be achieved by employing aqueous two-phase extraction (ATPE). Prior to fractionation, purification of betalains (devoid of sugars) was achieved employing ATPE comprising PEG 6000/ammonium sulphate. At a high tie line length (TLL) of 50.65%, phase volume ratio 1.96 and pH around 5, maximum differential partitioning was observed, wherein 92.62% of the betalains partitioned to the PEG-rich (top) phase while 72.38% of sugars partitioned to the salt-rich (bottom) phase. Fractionation of betalains into betacyanin and betaxanthin was achieved by multistage ATPE. At the end of the third stage ATPE, the bottom phase had only betaxanthin without any residual betacyanin. The fractions of betacyanin and betaxanthin were analyzed by spectrophotometric method and confirmed by HPLC.

Item Type:	Article
Uncontrolled Keywords:	Betalains Betacyanins Betaxanthins Aqueous two phase extraction Differential partitioning
Subjects:	600 Technology > 08 Food technology > 07 Food Engineering
Divisions:	Food Engineering
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	16 Oct 2014 09:35
Last Modified:	16 Oct 2014 09:35
URI:	http://ir.cftri.res.in/id/eprint/11659

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