Beta-Glucosidase Catalyzed Syntheses of Pyridoxine Glycosides.

Charles, Rajachristu Einstein and Divakar, S. (2009) Beta-Glucosidase Catalyzed Syntheses of Pyridoxine Glycosides. Bioscience Biotechnology and Biochemistry, 73 (1). pp. 233-236.

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Abstract

Enzymatic syntheses of pyridoxine glycosides were carried out in di-isopropyl ether organic medium using �beta-glucosidase isolated from sweet almond. Optimum conditions determined for the reaction with D-glucose were 40% (w/w D-glucose) �-glucosidase at 0.18mM (1.8 ml) of pH 5 acetate buffer over a 72 h incubation period. Of 11 carbohydrates employed, �-glucosidase gave 7-O-(�-D-glucopyranosyl)pyridoxine 5a, 7-O-(�-Dglucopyranosyl) pyridoxine 5b, 6-O-(�-D-glucopyranosyl) pyridoxine 5c, 7-O-(�-D-galactopyranosyl)pyridoxine 6a, 7-O-(�-D-galactopyranosyl)pyridoxine 6b, 6-O-(�-Dgalactopyranosyl) pyridoxine 6c, 7-O-(�-D-mannopyranosyl) pyridoxine 7a, 7-O-(�-D-mannopyranosyl)pyridoxine 7b, and 6-O-(�-D-mannopyranosyl)pyridoxine 7c in yields ranging from 23 to 40%.

Item Type:	Article
Uncontrolled Keywords:	beta�-glucosidase; glycosylation; pyridoxine; pyridoxine glycosides; 6-O and 7-O pyridoxine glycosides
Subjects:	500 Natural Sciences and Mathematics > 07 Life Sciences > 03 Biochemistry & Molecular Biology > 17 Vitamin Biochemistry
Divisions:	Fermentation Technology and Bioengineering
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	23 Jun 2017 06:16
Last Modified:	23 Jun 2017 06:16
URI:	http://ir.cftri.res.in/id/eprint/12648

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