Evaluation of the anti-dyslipidemic effect of spice fixed oils in the in vitro assays and the high fat diet-induced dyslipidemic mice.

Manasa, V. and Ajay, W. Tumaney (2022) Evaluation of the anti-dyslipidemic effect of spice fixed oils in the in vitro assays and the high fat diet-induced dyslipidemic mice. Food Bioscience, 46. p. 101574.

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Abstract

Dyslipidemia is a condition in which the lipid levels are altered in the body. The fixed oils (FOs) from spices, Alpinia galanga, Cinnamomum zeylanicum, Trigonella foenum-graecum, Foeniculum vulgare, and Myristica fragrans were evaluated as potential sources of anti-dyslipidemic activity. The nutraceutical compound fractions such as polyphenols, tocopherols, γ-oryzanol, and phytosterols were extracted from spice FOs, tested individually and in combination for cytotoxicity and their effect on lipid accumulation in 3T3-L1 and Hep-G2 cell lines. Further, the best performing FOs in the in vitro studies were fed to high fat diet-induced dyslipidemic C57BL/6 mice and studied its activity in the liver and adipose tissue. The in vitro assays showed no cytotoxicity in the studied FOs up to 200 μg/mL concentration. Out of all the studied nutraceutical compound fractions, the γ-oryzanol and phytosterols fractions showed a reduction in the accumulation of lipid droplets in 3T3-L1 cell lines. Moreover, the reconstituted nutraceutical fractions from C. zeylanicum, T. foenum-graecum, and F. vulgare FOs exhibited a dose dependent inhibition of lipid accumulation compared to the fractions of A. galanga and M. fragrans FOs. The in vivo studies confirmed the anti-dyslipidemic activity of C. zeylanicum FO that showed an improved lipid profile, antioxidant enzymes, and reduced lipid droplets in the liver and adipose tissue. The histopathological images of the liver and adipose tissue showed a comparable visualisation in Atorvastatin, and C. zeylanicum fed group mice. Hence, the spice FOs are potentially efficacious anti-dyslipidemic functional food ingredients and could be safer than statins.

Item Type:	Article
Uncontrolled Keywords:	Spice fixed oils γ-Oryzanol Anti-dyslipidemic activity C57BL/6 Oxidative stress markers
Subjects:	600 Technology > 08 Food technology > 30 Spices/Condiments
Divisions:	Dept. of Lipid Science
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	10 May 2022 09:11
Last Modified:	10 May 2022 09:11
URI:	http://ir.cftri.res.in/id/eprint/15243

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