Understanding the functional attributes of foodborne pathogen, Staphylococcus aureus RecA protein in homologous recombination and antibacterial resistance.

Kajal, Kiran (2022) Understanding the functional attributes of foodborne pathogen, Staphylococcus aureus RecA protein in homologous recombination and antibacterial resistance. Doctoral thesis, Central Food Technological Research Institute.

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Abstract

Foodborne diseases are caused by contamination and may occur at any stage of the food production, delivery, and consumption chain. Illness is caused by consuming food contaminated with bacteria, viruses, parasites, or chemical substances such as heavy metals. Foodborne diseases encompass a wide range of illnesses, from diarrhea to cancers. Staphylococcus aureus is the most dangerous pathogenic bacteria, among other staphylococcal bacterial strains. The recombinases present from primitive prokaryotes to higher eukaryotes. This recombinase is crucial in DNA transactions ( replication repair and recombination). RecA, the critical regulator protein in these processes, is highly conserved across all the organisms. To date, the role of RecA protein in S. aureus has not been studied. We report that the recombinant purified S. aureus RecA protein can show the canonical activities, including; DNA binding, displacement loop formation, strand exchange reaction, ATP- hydrolysis, and coprotease function. Further, our findings showed that the recombinant purified, anti�recombinase, S. aureus RecX protein was found to abolish S. aureus RecA activities in vitro. A

Item Type:	Thesis (Doctoral)
Uncontrolled Keywords:	Foodborne diseases, Staphylococcus aureus, RecA, regulator protein, antibiotic resistance
Subjects:	500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 29 Protein Chemistry 600 Technology > 08 Food technology > 10 Food Microorganisms
Divisions:	Food Microbiology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	19 May 2023 04:40
Last Modified:	19 May 2023 04:40
URI:	http://ir.cftri.res.in/id/eprint/16414

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