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Determination of bioaccessibility of beta-carotene in vegetables by in vitro methods.

Supriya, Veda and Akshaya, Kamath and Kalpana, Platel and Begum, Khyrunnisa and Srinivasan, K. (2006) Determination of bioaccessibility of beta-carotene in vegetables by in vitro methods. Molecular nutrition & food research, 50 (11). pp. 1047-52. ISSN 1613-4125

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The in vitro method in use for the determination of beta-carotene bioaccessibility involves simulated gastrointestinal digestion followed by ultracentrifugation to separate the micellar fraction containing bioaccessible beta-carotene and its quantitation. In this study, the suitability of two alternatives viz., membrane filtration and equilibrium dialysis were examined to separate the micellar fraction. Values of beta-carotene bioaccessibility obtained with the membrane filtration method were similar to those obtained by the ultracentrifugation method. Equilibrium dialysis was found not suitable for this purpose. Among the vegetables analyzed, fenugreek leaves had the highest content of beta-carotene (9.15 mg/100 g), followed by amaranth (8.17 mg/100 g), carrot (8.14 mg/100 g) and pumpkin (1.90 mg/100 g). Percent bioaccessibility of beta-carotene ranged from 6.7 in fenugreek leaves to 20.3 in carrot. Heat treatment of these vegetables by pressure cooking and stir-frying had a beneficial influence on the bioaccessibility of beta-carotene from these vegetables. The increase in the percent bioaccessibility of beta-carotene as a result of pressure-cooking was 100, 48 and 19% for fenugreek leaves, amaranth and carrot, respectively. Stir-frying in presence of a small quantity of oil led to an enormous increase in the bioaccessibility of beta-carotene from these vegetables, the increase being 263% (fenugreek leaves), 192% (amaranth leaves), 63% (carrot) and 53% (pumpkin).

Item Type: Article
Uncontrolled Keywords: Bioaccessibility / b-Carotene / Green leafy vegetables / Heat processing / Yellow-orange vegetables /
Subjects: 600 Technology > 08 Food technology > 23 Vegetables
Divisions: Dept. of Biochemistry
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 07 Aug 2008 07:12
Last Modified: 29 Aug 2018 06:54
URI: http://ir.cftri.res.in/id/eprint/1905

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