Isolation and characterization of a novel haemoprotein b-559 from Bengal gram (Cicer arietinum).

Ramadoss, C. S. and Shenoy, B. C. and Borthakur, A. (1987) Isolation and characterization of a novel haemoprotein b-559 from Bengal gram (Cicer arietinum). The Biochemical journal, 243 (3). pp. 723-8. ISSN 0264-6021

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Abstract

A haemoprotein was purified to apparent homogeneity from Bengal-gram seeds. The purified protein exhibited an absorption maximum at 412 nm (Soret band) that upon reduction with dithionite gave rise to a shift in the Soret band to 426 nm with concomitant appearance of an alpha-band at 559 nm and a beta-band at 530 nm. In the reduced state the Bengal-gram haemoprotein showed reactivity towards CO, nitrite and hydroxylamine. SDS/polyacrylamide-slab-gel electrophoresis showed that the haemoprotein has Mr 78,000. Gel-filtration and ultracentrifugal analyses suggest that the Bengal-gram haemoprotein is oligomeric in nature. Since it differs from photosynthetic membrane cytochrome b-559 in solubility in buffer, in reactivity towards CO and in molecular size, it appears to be a novel haemoprotein b-559.

Item Type:	Article
Uncontrolled Keywords:	haemoprotein, Bengal-gram seeds
Subjects:	500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 29 Protein Chemistry
Divisions:	Dept. of Biochemistry
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	26 Jul 2013 06:46
Last Modified:	26 Jul 2013 06:46
URI:	http://ir.cftri.res.in/id/eprint/2381

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