Intestinal epithelial cell alkaline phosphatase and Na+,K+-ATPase activities in albino rats in retinol deficiency.

Singh, K. R. and Krishnakantha, T. P. (1987) Intestinal epithelial cell alkaline phosphatase and Na+,K+-ATPase activities in albino rats in retinol deficiency. Nutrition Reports International, 35 (5). pp. 973-980.

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Abstract

Brush border and basolateral membranes were isolated from intestinal epithelial cell fractions of retinol-deficient and control rats. The activity of Na+,K+-ATPase was reduced in the villus fraction from retinol-deficient rats. Enzymic staining of alkaline phosphatase on 5% polyacrylamide gels showed a single activity band in both membrane fractions. Densitometric analysis of those bands showed a significant reduction only in the villus fraction of the brush border membranes from retinol-deficient rats. Gel electrophoretic analysis of brush border membranes and basolateral membranes in the presence of sodium dodecyl sulphate followed by periodate-Schiff's staining showed 5 and 4 bands, respectively, in all the villus, middle and crypt fractions. Among those bands only 1 band was affected in the villus fraction of both membranes.

Item Type:	Article
Uncontrolled Keywords:	Retinol-; deficiency-; alkaline-phosphatase; intestinal-mucosa; ADENOSINETRIPHOSPHATASE-
Subjects:	500 Natural Sciences and Mathematics > 07 Life Sciences > 03 Biochemistry & Molecular Biology > 17 Vitamin Biochemistry
Divisions:	Dept. of Biochemistry
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	29 Jul 2021 05:51
Last Modified:	29 Jul 2021 05:51
URI:	http://ir.cftri.res.in/id/eprint/8535

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