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Evaluation of Microalgal Biomass for Valuable Bioactive Metabolites

Labana, Saurav (2008) Evaluation of Microalgal Biomass for Valuable Bioactive Metabolites. [Student Project Report]

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This Dissertation / Report is the outcome of investigation carried out by the creator(s) / author(s) at the department/division of Central Food Technological Research Institute (CFTRI), Mysore mentioned below in this page.

Item Type: Student Project Report
Additional Information: Botryococcus species are known as a potential source for the production of lipids, hydrocarbons and other bioactive compounds of pharmaceutical and nutraceutical importance. So it is essential to explore and exploit the indigenous strains of Botryococcus species for production of lipids and other valuable bioactive metabolites, hence the present study focuses on the characterization of various indigenous strains of Botryococcus. Light microscopic and scanning electron microscopic studies have confirmed that all the indigenous strains of Botryococcus isolated from different locations were found to be colonial. RAPD marker analyses resulted in the amplification of totally 412 bands of 150–3000 bp, of which 390 bands were polymorphic, corresponding to nearly 95% genetic diversity. A strong linear relationship was observed between the Resolving power (Rp) of the primer and its ability to distinguish genotypes. Based on these data, a genetic similarity matrix was established and a dendrogram for each set of primers was developed by UPGMA. The genetic similarity coefficients in RAPD analysis ranged from 0.3302 to 0.7303. The study resulted in the identification and molecular classification of indigenous strains of Botryococcus. Nitrogen stress is found to increase carotenoids production in all the indigenous strains studied. MCRC strain is found to produce highest yields of total lipids followed by KS, Mamalla, DB-8, K and BRL. It is also clear that BRL is the lowest producer of lipids. Fatty acid profiles were also done by analyzing the FAMEs using GCMS. And it has been found that Dodecanoic acid (12:0), Tetradecanoic acid (14:0), Hexadecanoic acid (16:0), oleic (18:1), linoleic (18:2), and linolenic acids (18:3) are the major fatty acids produced by all the indigenous strains of Botryococcus.
Uncontrolled Keywords: Algae Botryococcus
Subjects: 500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology > 01 Algae
Divisions: Plant Cell Biotechnology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 15 Oct 2008 12:10
Last Modified: 28 Dec 2011 10:05
URI: http://ir.cftri.res.in/id/eprint/8762

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