Amyloglucosidase from Rhizopus mold and b-glucosidase from sweet almond catalysed syntheses of riboflavinyl glycosides

Sivakumar, Ramaiah and Divakar, S. (2009) Amyloglucosidase from Rhizopus mold and b-glucosidase from sweet almond catalysed syntheses of riboflavinyl glycosides. Indian Journal of Biotechnology, 8. pp. 400-407.

PDF IJBT%208%284%29%20400-407.pdf Restricted to Registered users only Download (106kB)

Abstract

Riboflavinyl glycosides were synthesized enzymatically using amyloglucosidase isolated from Rhizopus mold and b-glucosidase from sweet almond to produce more water soluble riboflavinyl derivatives. The reaction conditions were optimized in terms of incubation period, pH, buffer, enzyme and substrate concentrations. With D-glucose, D-galactose, D-mannose, D-ribose, sucrose, maltose and lactose, both the enzymes gave yields in the range of 5-40%. Amyloglucosidase catalysis gave C1a and C1b glycosides of D-glucose, D-galactose, D-mannose, D-ribose, maltose, C6-O-arylated products of D-glucose and maltose and C1-O-arylated product of sucrose. However, b-glucosidase gave C1b-glucoside, C1b-lactoside and C1a and C1b glycosides of D-galactose and D-mannose. Riboflavinyl glycosides showed angiotensin converting enzyme inhibitory activities, with IC50 values in the 0.8±0.04-2.08±0.1 mM range.

Item Type:	Article
Uncontrolled Keywords:	Amyloglucosidase, carbohydrates, b-glucosidase, regioselectivity, riboflavin, riboflavinyl glycosides, Rhizopus, sweet almond
Subjects:	600 Technology > 08 Food technology > 16 Nutritive value > 01 Carbohydrates 600 Technology > 08 Food technology > 33 Nuts
Divisions:	Fermentation Technology and Bioengineering
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	15 Nov 2010 06:44
Last Modified:	28 Dec 2011 10:19
URI:	http://ir.cftri.res.in/id/eprint/9780

Actions (login required)

View Item