Influence of calcium channel modulators in capsaicin production by cell suspension cultures of Capsicum frutescens Mill.
Sudha, G. and Ravishankar, G. A. (2002) Influence of calcium channel modulators in capsaicin production by cell suspension cultures of Capsicum frutescens Mill. Current Science, 83 (4). pp. 480-484.
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Abstract
The production of capsaicin by cell suspension cultures of Capsicum frutescens mediated through the calcium channel was studied. Administration of the calcium ionophore A23187 resulted in a 1.43-fold enhancement of the total capsaicin production in the cell suspension cultures. Treatments wherein the calcium channel modulators verapamil and chlorpromazine were administered resulted in lower growth and capsaicin production, suggesting that calcium is involved in the signal transduction of capsaicin pathway in the suspension culture. The activity of Ca2+ATPase, which is a calmodulin-related enzyme, was also studied under the influence of the calcium channel modulators. Maximum activity of Ca2+ATPase was observed preceding the capsaicin elicitation. The activity of capsaicin synthase correlated with the increased capsaicin levels under the influence of calcium ionophore.
Item Type: | Article |
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Uncontrolled Keywords: | Verapamil; Chlorpromazine; Calmodulin-related enzyme |
Subjects: | 600 Technology > 08 Food technology > 30 Spices/Condiments |
Divisions: | Plant Cell Biotechnology |
Depositing User: | Somashekar K.S |
Date Deposited: | 21 Jun 2011 09:46 |
Last Modified: | 28 Dec 2011 10:24 |
URI: | http://ir.cftri.res.in/id/eprint/10142 |
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