Antibacterial Activity of Nigella sativa L. Seed Extracts.

Suresh Kumar, T. V. and Negi, P. S. and Udaya Sankar, K. (2010) Antibacterial Activity of Nigella sativa L. Seed Extracts. British Journal of Pharmacology and Toxicology, 1 (2). pp. 96-100. ISSN 2044-2467

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Abstract

Most of the bacterial pathogens are resistant to existing synthetic antibacterial agents demanding an increasing effort to seek effective phytochemicals as antibacterial agents against such pathogens. Nigella sativa L. (black cumin) seeds play an important role in folk medicine and some of its major constituents are reported to be pharmacologically active. In this present work, black cumin seed extracts were obtained using supercritical carbon dioxide (SCCO2) and conventional soxtec extraction using various organic solvents. The antibacterial activities of the extracts were investigated by the agar dilution method against Gram-positive bacteria (Bacillus cereus F 4810 and Staphylococcus aureus FRI 722) and Gram-negative bacteria (Escherichia coli MTCC 108 and Yersinia enterocolitica MTCC 859). SCCO2-1 (120 bar/40ºC) extract showed effective growth inhibition than conventional solvent extracts against all the tested bacteria. Further the antibacterial principle present in the extract was isolated and characterized found to be thymoquinone.

Item Type:	Article
Uncontrolled Keywords:	Agar dilution method, black cumin, 1H and 13C NMR, supercritical CO2 extraction, thymoquinone
Subjects:	600 Technology > 08 Food technology > 30 Spices/Condiments
Divisions:	Human Resource Development
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	19 Jan 2012 04:12
Last Modified:	28 Dec 2016 12:55
URI:	http://ir.cftri.res.in/id/eprint/10511

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