Diffusion, Frowning and Smiling of Low Molecular Weight Protein Bands: A Simple, Rapid and Efficient Solution

Rajagopal, Kammara and Chandana, Thimme Gowda and Praveen Kumar, Singh (2015) Diffusion, Frowning and Smiling of Low Molecular Weight Protein Bands: A Simple, Rapid and Efficient Solution. International Journal of Peptide Research and Therapeutics, 21 (1). pp. 7-11.

PDF International Journal of Peptide Research and Therapeutics March 2015, Volume 21, Issue 1, pp 7-11.pdf - Published Version Restricted to Registered users only Download (221kB)

Abstract

A modified method of SDS-polyacrylamide gel electrophoresis for the efficient separation of low molecular weight proteins (ranging 1.4–10 kDa, containing disulfide bonds) without frowning, smiling and diffusion is described. SDS-polyacrylamide and tricine gel electrophoresis are considered as standard approaches for low molecular weight protein separation, despite frowning, smiling and diffusion problems. These methods are inefficient and confusing as Coomassie brilliant blue stained low molecular weight protein bands are difficult to identify since proteins get diffused and, it is difficult to identify as proteins. The method developed offers a solution to the existing problem and is more suitable for the efficient separation of low molecular weight proteins with high resolution (with di-sulfide bonds).

Item Type:	Article
Uncontrolled Keywords:	Tricine gel · SDS-polyacrylamide · Frowning · Smiling · Diffusion · Low-molecular weight polypeptides
Subjects:	500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 29 Protein Chemistry
Divisions:	Protein Chemistry and Technology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	18 May 2016 06:41
Last Modified:	30 Dec 2016 08:32
URI:	http://ir.cftri.res.in/id/eprint/12149

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