ROG1 encodes a monoacylglycerol lipase in Saccharomyces cerevisiae

Varthini, Lakshmanaperumal Vishnu and Selvaraju, Kandasamy. and Malathi, S. and Vasanthi, Nachiappan (2015) ROG1 encodes a monoacylglycerol lipase in Saccharomyces cerevisiae. FEBS Letters, 589. pp. 23-30.

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Abstract

Lipid metabolism is extensively studied in Saccharomyces cerevisiae. Here, we report that revertant of glycogen synthase kinase mutation-1 (Rog1p) possesses monoacylglycerol (MAG) lipase activity in S. cerevisiae. The lipase activity of Rog1p was confirmed in two ways: through analysis of a strain with a double deletion of ROG1 and monoglyceride lipase YJU3 (yju3Drog1D) and by site-directed mutagenesis of the ROG1 lipase motif (GXSXG). Rog1p is localized in both the cytosol and the nucleus. Overexpression of ROG1 in a ROG1-deficient strain resulted in an accumulation of reactive oxygen species. These results suggest that Rog1p is a MAG lipase that regulates lipid homeostasis.

Item Type:	Article
Uncontrolled Keywords:	Lipid metabolism Monoacylglycerol Lipid droplet Reactive oxygen species Monoacylglycerol lipase Saccharomyces cerevisiae
Subjects:	500 Natural Sciences and Mathematics > 07 Life Sciences > 03 Biochemistry & Molecular Biology > 11 Lipid Biochemistry 500 Natural Sciences and Mathematics > 07 Life Sciences > 03 Biochemistry & Molecular Biology > 19 Yeast
Divisions:	Lipid Science and Traditional Foods
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	30 May 2016 07:45
Last Modified:	30 May 2016 07:45
URI:	http://ir.cftri.res.in/id/eprint/12215

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