Effect of Different Carbon Sources on the Growth of Alpha-HCH Degrading Pseudomonas Ptm+ Strain

Anu Appaiah, K. A. and Karanth, N. G. K. (1996) Effect of Different Carbon Sources on the Growth of Alpha-HCH Degrading Pseudomonas Ptm+ Strain. Journal of Soil Biology and Ecology, 16 (2). pp. 103-107.

PDF J.Soil Biol Ecol 16(2) 103-107(1996).pdf - Published Version Restricted to Registered users only Download (109kB)

Abstract

A bacterial isolate capable of degrading alpha-isomer of the insecticide HCH used this chemical as the sole source of carbon and energy. Pseudomonas (Ptm' strain) produced 10 times more biomass when mannitol was the carbon source and 3 times more biomass with glucose. Binary mixtures of mannitol nnd alpha·HCH showed simultaneous utilisation of both the molecules. Pattern of proliferation on HCH and its structural analog inositol was similar indicating the involvement of common enzyme systems in their breakdown. Utilisation of alpha-HCH and yeast extract increased when both substrates were added together to the medium, suggesting some co-factors contained in yeast extract accelerated alpha HCH degradation in Ptm+ strain, resulting in better growth. Mannitol (11.1 mM) yeast extract (0.25%) and HCH (68.8 uM) containing medium was suitable for the biomass production of HCH degrading bacteria.

Item Type:	Article
Uncontrolled Keywords:	bacterial isolate insecticide HCH Pseudomonas
Subjects:	500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 26 Pesticide Chemistry
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	04 Jan 2017 12:11
Last Modified:	04 Jan 2017 12:11
URI:	http://ir.cftri.res.in/id/eprint/12607

Actions (login required)

View Item