Influence of Metal Ions on Dehydrohalogenase Activity.

Latha, R. and Mandappa, I. M. and Thakur, M. S. and Manonmani, H. K. (2011) Influence of Metal Ions on Dehydrohalogenase Activity. African Journal of Basic and Applied Sciences, 3 (2). pp. 45-51.

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Abstract

The effect of few metal ions on DDT-and HCH-dehydrohalogenase activity was studied. Both the enzymes were inactivated in the presence of these metals. The apparent rate constants of inactivation at different metal concentrations and conformational changes in the presence of 100mM metals were obtained. The conformational changes of both the enzymes were followed by fluorescence spectra and circular dichoism spectra. Comparison of the results of both the enzymes indicated that both the enzymes were inactivated by the addition of metal iOl13. There was a progressive loss in catalytic activity of dehydrohalogenase enzymes with increasing concentrations of metals. Both dehydrohalogenases studied had almost the same effect The fluorescence spectrum of both the enzymes exposed to metal ions showed a shift towards the red region. The CD spectrum of these metal treated enzymes showed significant changes such as reduction in a-helices and ~-sheets_ Alkali metals, alkaline earth metals, heavy metals and transition metals studied had almost the same changes in the secondary structure of both dehydrohalogenases.

Item Type:	Article
Uncontrolled Keywords:	Confomlatlonal change . DDT- dehydrohalogenase HCH dehydrohalogenase, Inactivation, Metal ions
Subjects:	600 Technology > 05 Chemical engineering > 03 Enzyme Biotechnology and Engineering
Divisions:	Fermentation Technology and Bioengineering Food Safety Analytical Quality Control Lab
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	28 Jun 2017 05:56
Last Modified:	28 Jun 2017 05:56
URI:	http://ir.cftri.res.in/id/eprint/12687

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