Modulation of K+ transport across synaptosomes of rat brain by synthetic pyrethroids

Valeswara Rao, Gazula and Jagannatha Rao, Kosagi Sharaf (1997) Modulation of K+ transport across synaptosomes of rat brain by synthetic pyrethroids. Journal of Neurological Sciences (147). pp. 127-133.

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Abstract

Potassium transport across synaptosomes was studied under the influence of two synthetic pyrethroids, (Permethrin, without the cyano group) and Cypermethrin (CPM, with the cyano group). Synaptosomes were isolated from rat brain cerebral cortex and incubated with 40 PM of PM and CPM for 15 min at 37°C. K’ release was monitored by a K’-sensitive electrode. CPM caused more K+ release from synaptosomes compared to PM. K+ transport is regulated by Naf-K’-ATPase, K+-ATPase and Kt channels. To understand the mode of action, synaptosomes were preincubated with 9.5X l0-5 M ouabain (inhibitor of Na’-K+-ATPase), 1.7X lOA* M N-ethylmaleamide (K’-ATPase inhibitor), and 9.5X 10-5 M quine sulfate (K+-channel blocker) for 15 min at 37°C. In the presence of ouabain and N-ethylmaleamide, PM- and CPM-induced K’ release was decreased and in the presence of quinine sulfate, there was no release of K’. Furthermore, the studies indicated that PM and CPM significantly decreased K’ uptake.

Item Type:	Article
Uncontrolled Keywords:	Permethrin; Cypermethrin; Kt transport; Synaptosomes
Subjects:	500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 26 Pesticide Chemistry
Divisions:	Dept. of Biochemistry
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	04 Jul 2007 09:06
Last Modified:	28 Dec 2011 09:28
URI:	http://ir.cftri.res.in/id/eprint/1288

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