Gold nanoparticles based enzyme biosensor for the detection of chloramphenicol.

Richa, Sharma and Akshath, U. S. and Praveena, Bhatt and Thakur, M. S. and Raghavarao, K. S. M. S. (2017) Gold nanoparticles based enzyme biosensor for the detection of chloramphenicol. Procedia Technology, 27. 282 -286.

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Abstract

Chronic use of chloramphenicol (CAM) antibiotic leads to anaemia and bone marrow suppression resulting in 40 – 50% mortality. Hence, there is a need to develop an economical, fast and convenient method to detect CAM in milk, honey, shrimp and other aquaculture products. In the current method, coenzyme A was used to indirectly quantify CAM (since it is the cofactor product of the acetylation reaction of CAM). Coenzyme A (CoASH) was used to stabilize gold nanoparticles which were characterized by studying their extinction spectra. The reductant concentration and synthesis time were optimized. With optimized parameters the proposed system could detect CoASH up to 0.1 nM in buffer, with a linear range of detection from 0.1 μM to 1 mM.

Item Type:	Article
Uncontrolled Keywords:	Chloramphenicol; gold nanoparticles; enzyme biosensor; coenzyme A; chloramphenicol acetyltransferase
Subjects:	500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 11 Antibiotics Chemistry 600 Technology > 05 Chemical engineering > 03 Enzyme Biotechnology and Engineering
Divisions:	Fermentation Technology and Bioengineering Food Engineering
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	01 Feb 2018 04:11
Last Modified:	01 Feb 2018 04:11
URI:	http://ir.cftri.res.in/id/eprint/13390

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