Optimised production and utilisation of exopolysaccharide from Agrobacterium radiobacter

Triveni, R. and Shamala, T. R. and Rastogi, N. K. (2001) Optimised production and utilisation of exopolysaccharide from Agrobacterium radiobacter. Process Biochemistry, 36. pp. 787-795.

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Abstract

The cultural conditions for bacterial exopolysaccharide production by Agrobacterium radiobacter were optimised by response surface methodology (RSM) to maximise the viscosity of the broth and the yield of the polysaccharide. A study of complex interactions among sucrose concentration (0.5–5.5 g%), inoculum concentration (1–15%, v:v) and pH (4–8) was carried out using central composite design of experiments. The exopolysaccharide contained glucose, galactose and rhamnose (88.9:10.4:0.7) and formed an elastic gel on heating followed by cooling, at 2 g% concentration. The exopolysaccharide produced in a simpler inorganic nitrogen containing medium can be used as an emulsifier, viscosifier and also as a gelling agent in the preparation of microbiological media. The optimum conditions, which were obtained for maximum viscosity of the broth (105.64 mPas) and the yield of the polysaccharide (2.26%) were, sucrose concentration 2.62%, inoculum concentration 1.0% and pH 6.24.

Item Type:	Article
Uncontrolled Keywords:	Bacterial gelling polysaccharide; Emulsifier; Optimisation; Response surface methodology; Central composite rotatable design
Subjects:	500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology 500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 28 Polysaccharide Chemistry
Divisions:	Food Engineering Food Microbiology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	03 Oct 2007 11:15
Last Modified:	10 Oct 2018 11:19
URI:	http://ir.cftri.res.in/id/eprint/1464

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