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Crosstalk between protein N-glycosylation and lipid metabolism in Saccharomyces cerevisiae.

Antonisamy, William James and Ravi, C. and Malathi, Srinivasan and Vasanthi, Nachiappan (2019) Crosstalk between protein N-glycosylation and lipid metabolism in Saccharomyces cerevisiae. Scientific Reports, 9. p. 14485.

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The endoplasmic reticulum (ER) is a multi functional organelle and plays a crucial role in protein folding and lipid biosynthesis. The SEC59 gene encodes dolichol kinase, required for protein glycosylation in the ER. The mutation of sec59-1 caused a protein N-glycosylation defect mediated ER stress resulting in increased levels of phospholipid, neutral lipid and sterol, whereas growth was reduced. In the sec59-1∆ cell, the N-glycosylation of vacuolar carboxy peptidase-Y (CPY) was signifcantly reduced; whereas the ER stress marker Kar2p and unfolded protein response (UPR) were signifcantly increased. Increased levels of Triacylglycerol (TAG), sterol ester (SE), and lipid droplets (LD) could be attributed to upregulation of DPP1, LRO1, and ARE2 in the sec 59-1∆ cell. Also, the diacylglycerol (DAG), sterol (STE), and free fatty acids (FFA) levels were signifcantly increased, whereas the genes involved in peroxisome biogenesis and Pex3-EGFP levels were reduced when compared to the wild-type. The microarray data also revealed increased expression of genes involved in phospholipid, TAG, fatty acid, sterol synthesis, and phospholipid transport resulting in dysregulation of lipid homeostasis in the sec59-1∆ cell. We conclude that SEC59 dependent N-glycosylation is required for lipid homeostasis, peroxisome biogenesis, and ER protein quality control.

Item Type: Article
Uncontrolled Keywords: lipid biosynthesis, Saccharomyces cerevisiae, SEC59 gene
Subjects: 500 Natural Sciences and Mathematics > 07 Life Sciences > 03 Biochemistry & Molecular Biology > 11 Lipid Biochemistry
Divisions: Dept. of Lipid Science
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 25 Nov 2020 11:07
Last Modified: 25 Nov 2020 11:07
URI: http://ir.cftri.res.in/id/eprint/14646

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