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A cost-efective polyethylene glycol-based method for the isolation of functional edible nanoparticles from ginger rhizomes.
Sreeram, P. K. and Durga, Prasad and Ravi, Kasiappan and Sachin, R. Chaudhari and Gopinath, M. S. (2020) A cost-efective polyethylene glycol-based method for the isolation of functional edible nanoparticles from ginger rhizomes. Scientific Reports, 10. p. 4456.
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Abstract
Edible nanoparticles (ENPs) are nano-sized vesicles derived from edible plants. These ENPs are loaded with plant derived microRNAs, protein, lipids and phytochemicals. Recently, ginger derived ENPs was shown to prevent infammatory bowel diseases and colon cancer, in vivo, highlighting their therapeutic potential. Conventionally, diferential centrifugation with an ultra-centrifugation step is employed to purify these ENPs which imposes limitation on the cost-efectiveness of their purifcation. Herein, we developed polyethylene glycol-6000 (PEG6000) based ginger ENP purifcation (PEG-ENPs) method, which eliminates the need for expensive ultracentrifugation. Using diferent PEG6000 concentrations, we could recover between 60% to 90% of ENPs compared to ultracentrifugation method. PEG-ENPs exhibit near identical size and zeta potential similar to ultra-ENPs. The biochemical composition of PEGENPs, such as proteins, lipids, small RNAs and bioactive content is comparable to that of ultra-ENPs. In addition, similar to ultra-ENPs, PEG-ENPs are efciently taken up by the murine macrophages and protects cells from hydrogen peroxide induced oxidative stress. Since PEG has been approved as food additive, the PEG method described here will provide a cost-efective alternative to purify ENPs, which can be directly used as a dietary supplement in therapeutic formulations.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | edible plants, Edible nanoparticles, ginger |
| Subjects: | 600 Technology > 08 Food technology > 30 Spices/Condiments |
| Divisions: | Dept. of Biochemistry |
| Depositing User: | Food Sci. & Technol. Information Services |
| Date Deposited: | 25 Nov 2020 11:14 |
| Last Modified: | 25 Nov 2020 11:14 |
| URI: | http://ir.cftri.res.in/id/eprint/14647 |
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