Application of Optimized and Validated Agar Overlay TLC–Bioautography Assay for Detecting the Antimicrobial Metabolites of Pharmaceutical Interest.

Bettadapura Rameshgowda, Nuthan and Devaraju, Rakshith and Kuppuru Mallikarjunaiah, Marulasiddaswamy and Yashavantha Rao, H.C. and Ramesha, Kolathur Puttamadaiah and Chandra Mohana, Nagabhushana and Siddappa, Shiva and Darshan, Dorairaj (2020) Application of Optimized and Validated Agar Overlay TLC–Bioautography Assay for Detecting the Antimicrobial Metabolites of Pharmaceutical Interest. Journal of Chromatographic Science. pp. 1-10.

PDF Journal of Chromatographic Science, 2020, 1–10.pdf - Published Version Restricted to Registered users only Download (1MB) | Request a copy

Abstract

The agar overlay TLC–bioautography is one of the crucial methods for simultaneous in situ detection and separation of antimicrobial metabolites of pharmaceutical interest. The main focus of this research relies on the dereplication of an antimicrobial metabolite coriloxin derived from mycoendophytic Xylaria sp. NBRTSB-20 with a validation of agar overlay TLC–bioautography technique. This polyketide metabolite coriloxin was purified by column chromatography, and its purity was assessed by HPLC, UPLC-ESI-QTOF-MS, FT-IR and NMR spectral analysis. The antimicrobial capability of ethyl acetate extract and the purified compound coriloxin was determined by disc diffusion, minimal inhibitory concentration and agar overlay TLC–bioautography assay. The visible LOD of coriloxin antimicrobial activity was found at 10 µg for Escherichia coli and 20 µg for both Staphylococcus aureus and Fusarium oxysporum. Inter- and intra-day precision was determined as the relative standard deviation is less than 6.56%, which proved that this method was precise. The accuracy was expressed as recovery, and the values were found ranging from 91.18 to 108.73% with RSD values 0.94–2.30%, respectively. The overall findings of this investigation suggest that agar overlay TLC–bioautography assay is a suitable and acceptable method for the in situ determination of antimicrobial pharmaceuticals

Item Type:	Article
Uncontrolled Keywords:	antimicrobial metabolites, pharmaceutical, coriloxin, TLC–bioautography technique, polyketide metabolite
Subjects:	500 Natural Sciences and Mathematics > 10 Plants 500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 06 Chromatography
Divisions:	Plant Cell Biotechnology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	02 Dec 2020 11:17
Last Modified:	02 Dec 2020 11:17
URI:	http://ir.cftri.res.in/id/eprint/14670

Actions (login required)

View Item