Molecular advancements on over-expression, stability and catalytic aspects of endo-b-mannanases.

Gaurav Singh, Kaira and Mukesh, Kapoor (2021) Molecular advancements on over-expression, stability and catalytic aspects of endo-b-mannanases. Critical Reviews in Biotechnology, 41 (1). pp. 1-15. ISSN 0738-8551

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Abstract

The hydrolysis of mannans by endo-b-mannanases continues to gather significance as exemplified by its commercial applications in food, feed, and a rekindled interest in biorefineries. The present review provides a comprehensive account of fundamental research and fascinating insights in the field of endo-b-mannanase engineering in order to improve over-expression and to decipher molecular determinants governing activity-stability during harsh conditions, substrate recognition, polysaccharide specificity, endo/exo mode of action and multi-functional activities in the modular polypeptide. In-depth analysis of the available literature has also been made on rational and directed evolution approaches, which have translated native endo-b-mannanases into superior biocatalysts for satisfying industrial requirements.

Item Type:	Article
Uncontrolled Keywords:	Endo-b-mannanase; overexpression; functional dissection; enzyme stability; substrate hydrolysis; rational designing; directed evolution
Subjects:	500 Natural Sciences and Mathematics > 07 Life Sciences > 03 Biochemistry & Molecular Biology > 07 Enzyme Biochemistry
Divisions:	Protein Chemistry and Technology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	30 Dec 2020 10:50
Last Modified:	30 Dec 2020 10:50
URI:	http://ir.cftri.res.in/id/eprint/14719

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