Degradation of Salicylic Acid by Free and Immobilized Cells of Pseudomonas sp. Strain NGK1.

Neelakanteshwar Patil, K. and Sharanagouda, U. and Javed, H. Niazi (2003) Degradation of Salicylic Acid by Free and Immobilized Cells of Pseudomonas sp. Strain NGK1. Journal of Microbiology and Biotechnology, 13 (1). pp. 29-34. ISSN 1017-7825

PDF J. Microbiol. Biotechnol. (2003), 13(1), 29–34.pdf - Published Version Restricted to Registered users only Download (88kB)

Abstract

A Pseudomonas sp. strain NGK1 (NCIM 5120) capable of utilizing salicylate was immobilized in alginate and polyurethane foam (PUF). The degradation rate of salicylate by freely suspended cells was compared with the degradation rate by immobilized cells. In an initial 20 and 40 mM salicylate, free cells (2×1011 cfu ml-1 ) degraded to 16 and 14 mM, alginateentrapped cells degraded to 18 and 26 mM, and PUF-entrapped cells degraded to 20 and 32 mM salicylate, respectively, in batch cultures. The alginate- and PUF-entrapped cells were used in repeated batch and continuous culture systems. The efficiency of both the immobilized systems for the degradation of salicylate was compared. It has been observed that the PUF-entrapped cells could be reused for more than 20 cycles whereas alginate-entrapped cells could be reused for a maximum of only 12 cycles, after which a decrease in degradation rate was observed with the initial 20 and 40 mM salicylate. The continuous degradation of salicylate by freely suspended cells showed a negligible degradation rate of salicylate when compared with immobilized cells. With the immobilized cells in both alginate and polyurethane foam, the degradation rate increased with an increase in the dilution rate up to 2 h-1 for 20 mM, and 1.5 h-1 for 40 mM salicylate. The results revealed that PUF-entrapped cells were more efficient for the degradation of salicylate than alginate-entrapped cells and freely suspended cells

Item Type:	Article
Uncontrolled Keywords:	Degradation, immobilization, salicylate, polyurethane foam, alginate, Pseudomonas sp. strain NGK1
Subjects:	500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	01 Jan 2021 08:39
Last Modified:	01 Jan 2021 08:39
URI:	http://ir.cftri.res.in/id/eprint/14747

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