In-silico analysis of Covid-19 genome sequences of Indian origin: impact of mutations in identification of SARS-Co-V2.

Kabilan, Mani and Kannan, Thirumalmuthu and Divya, Sri Kathiresan (2021) In-silico analysis of Covid-19 genome sequences of Indian origin: impact of mutations in identification of SARS-Co-V2. Molecular and Cellular Probes, 58. p. 101748. ISSN 0890-8508

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Abstract

Covid-19 disease caused by SARS-CoV-2 is still being transmitted in developed and developing countries irrespective of healthcare setups. India with 1.3 billion people in the world is severely affected by Covid-19 with 11.3 million cases and 157 000 deaths so far. We have assessed the mismatches in WHO recommended rRT-PCR assays primer and probe binding regions against SARS-CoV-2 Indian genome sequences through in-silico bioinformatics analysis approach. Primers and probe sequences belonging to CN-CDC-ORF1ab from China and HKU-ORF1b from Hong Kong targeting ORF1ab gene while NIH-TH-N from Thailand, HKU-N from Hong Kong and US-CDCN-2 from USA targeting N genes displayed accurate matches (>98.3%) with the 2019 novel corona virus sequences from India. On the other hand, none of the genomic sequences displayed exact match with the primer/ probe sequences belonging to Charit´e-ORF1b from Germany targeting ORF1ab gene.

Item Type:	Article
Uncontrolled Keywords:	Covid-19 Primer and probe mismatches rRT-PCR Indian SARS-CoV-2 genome sequences
Subjects:	500 Natural Sciences and Mathematics > 07 Life Sciences > 03 Biochemistry & Molecular Biology
Divisions:	Dept. of Lipid Science
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	29 Nov 2021 04:36
Last Modified:	29 Nov 2021 04:36
URI:	http://ir.cftri.res.in/id/eprint/15100

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