Genome shuffling for phenotypic improvement of industrial strains through recursive protoplast fusion technology.

Ravichandra, Hospet and Devarajan, Thangadurai and Natália, Cruz Martins and Jeyabalan, Sangeetha and Anu Appaiah, K. A. (2021) Genome shuffling for phenotypic improvement of industrial strains through recursive protoplast fusion technology. Critical Reviews in Food Science and Nutrition. pp. 1-10. ISSN 1549-7852

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Abstract

Strains’ improvement technology plays an essential role in enhancing the quality of industrial strains. Several traditional methods and modern techniques have been used to further improve strain engineering programs. The advances stated in strain engineering and the increasing demand for microbial metabolites leads to the invention of the genome shuffling technique, which ensures a specific phenotype improvement through inducing mutation and recursive protoplast fusion. In such technique, the selection of multi-parental strains with distinct phenotypic traits is crucial. In addition, as this evolutionary strain improvement technique involves combinative approaches, it does not require any gene sequence data for genome alteration and, therefore, strains developed by this elite technique will not be considered as genetically modified organisms. In this review, the different stages involved in the genome shuffling technique and its wide applications in various phenotype improvements will be addressed. Taken together, data discussed here highlight that the use of genome shuffling for strain improvement will be a plus for solving complex phenotypic traits and in promoting the rapid development of other industrially important strains.

Item Type:	Article
Uncontrolled Keywords:	Genome shuffling; microbial breeding; mutagenesis; recursive protoplast fusion; strain improvement
Subjects:	500 Natural Sciences and Mathematics > 07 Life Sciences > 03 Biochemistry & Molecular Biology
Divisions:	Food Microbiology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	29 Mar 2022 04:46
Last Modified:	29 Mar 2022 04:46
URI:	http://ir.cftri.res.in/id/eprint/15141

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