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Rational design of hyperstable antibacterial peptides for food preservation.

Yashavantha, L. Vishweshwaraiah and Abhishek, Acharya and Vinayak, Hegde and Balaji, Prakash (2021) Rational design of hyperstable antibacterial peptides for food preservation. NPJ Science of Food, 5 (26).

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We describe the design of peptides with properties like thermostability, pH stability, and antibacterial activity against a few bacterial food pathogens. Insights obtained from classical structure-function analysis of natural peptides and their mutants through antimicrobial and enzymatic assays are used to rationally develop a set of peptides. pH and thermostability assays were performed to demonstrate robust antimicrobial activity post-treatment with high temperatures and at wide pH ranges. We have also investigated the mode of action of these hyperstable peptides using membrane permeability assays, electron microscopy, and molecular dynamics simulations. Notably, through mutational studies, we show that these peptides elicit their antibacterial action via both membrane destabilization and inhibition of intracellular trypsin—the two functions attributable to separate peptide segments. Finally, toxicity studies and food preservation assays demonstrate the safety and efficacy of the designed peptides for food preservation. Overall, the study provides a general ‘blueprint’ for the development of stable antimicrobial peptides (AMPs). Insights obtained from this work may also be combined with combinatorial methods in high-throughput studies for future development of antimicrobials for various applications.

Item Type: Article
Uncontrolled Keywords: peptides, bacterial food pathogens
Subjects: 500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 25 Peptide Chemistry
600 Technology > 08 Food technology > 29 Microbiological food
Divisions: Molecular Nutrition
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 29 Mar 2022 10:24
Last Modified: 29 Mar 2022 10:24
URI: http://ir.cftri.res.in/id/eprint/15158

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