Abundant class III acidic chitinase homologue in tamarind (Tamarindus indica) seed serves as the major storage protein.

Rao, Devavratha H and Gowda, Lalitha R (2008) Abundant class III acidic chitinase homologue in tamarind (Tamarindus indica) seed serves as the major storage protein. Journal of agricultural and food chemistry, 56 (6). pp. 2175-2182. ISSN 0021-8561

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Abstract

The phyla Leguminosae contains protease inhibitors, lectins, chitinases, and glycohydrolases as major defense proteins in their seeds. Electrophoretic analysis of the seed proteins of tamarind ( Tamarindus indica L.), an agri-waste material, indicated the unusual presence of two major proteins comparable to overexpression of recombinant proteins. These proteins were identified by amino-terminal analysis to be (1) Kunitz-type trypsin inhibitor and (2) class III endochitinase (34000 Da). These two proteins were purified to apparent homogeneity by a single-step chitin bead affinity chromatography and characterized. The Kunitz inhibitor was specific toward inhibiting trypsin with a stoichiometry of 1:1. The 33000 +/- 1000 Da protein, accounting for >50% of the total seed protein, is an acidic glycoprotein exhibiting a very low endotype hydrolytic activity toward chitin derivatives. SDS-PAGE followed by densitometry of tamarind seed germination indicates the disappearance of the chitinase with the concomitant appearance of a cysteine endopeptidase. On the basis of its abundance, accumulation without any pathogenesis-related stimulus, temporal regulation, amino acid composition, and very low enzyme activity, this 34000 Da protein designated "tamarinin" physiologically serves as the major storage protein.

Item Type:	Article
Uncontrolled Keywords:	Tamarinin; germination; Kunitz-type trypsin inhibitor; kernel protein; chitin bead affinity chromatography; densitometry
Subjects:	500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 29 Protein Chemistry 600 Technology > 08 Food technology > 30 Spices/Condiments
Divisions:	Protein Chemistry and Technology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	08 Aug 2008 10:32
Last Modified:	28 Dec 2011 09:35
URI:	http://ir.cftri.res.in/id/eprint/1809

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