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In vitro studies on liposomal amphotericin B obtained by supercritical carbon dioxide-mediated process.
Udaya Sankar, K. and Deepan Raja, B. and Usha Rani, G. and Manohar, B. and Venkateswaran, G. (2007) In vitro studies on liposomal amphotericin B obtained by supercritical carbon dioxide-mediated process. Nanomedicine : nanotechnology, biology, and medicine, 3 (4). pp. 273-80. ISSN 1549-9642
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Abstract
Nanotechnology in drug delivery is a rapidly expanding field. Nanosized liposomal preparations are already in use for efficient drug delivery with better therapeutic indices. Existing methods of liposome preparation are limited by problems of scale-up, difficulty in controlling size, and intercalation efficiency. Here we prepare amphotericin B-intercalated liposomes by a novel process where amphotericin B and purified phosphatidyl choline are solubilized in suitable solvent and precipitated in supercritical fluid carbon dioxide (known as a gas antisolvent technique), to obtain microsized particles that are subsequently introduced into a buffer solution. The morphology of liposomes was characterized through a phase-contrast microscope, and the particle size distribution studied by laser technique showed nanosize with a narrow range of size distribution (between 0.5 and 15 microm) and a higher intercalation efficiency. In vitro studies conducted using Aspergillus fumigatus (MTCC 870) strain proved to be efficient in the retardation of the growth of the organism.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Supercritical; Carbon dioxide; Gas antisolvent; Liposomes; Amphotericin B; In vitro studies |
| Subjects: | 600 Technology > 02 Engineering & allied operations |
| Divisions: | Food Engineering Food Microbiology |
| Depositing User: | Food Sci. & Technol. Information Services |
| Date Deposited: | 18 Aug 2008 11:36 |
| Last Modified: | 23 May 2012 07:10 |
| URI: | http://ir.cftri.res.in/id/eprint/1828 |
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