Guar Gum β-Manno-Oligosaccharides Alleviate Ulcerative Colitis in Mice Models via Gut Microflora Modulation

Ashwini, M. and Ajit, Antony and Shilpa, H. S. and Ravi Mangu, S. V. V. S. and Kunal, Sharan and Mukesh, Kapoor (2025) Guar Gum β-Manno-Oligosaccharides Alleviate Ulcerative Colitis in Mice Models via Gut Microflora Modulation. Journal of Dietary Supplements, 22 (5). pp. 750-774.

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Abstract

Gut dysbiosis is a key feature in ulcerative colitis, a globally prevalent subtype of inflammatory bowel disease. Microbiota-directed dietary supplements can restore the dysbiotic gut and alleviate ulcerative colitis. We aimed to delineate whether prebiotic guar gum β-manno-oligosaccharides [GG-β-MOs(i) 500 mg/kg, (ii) 1000 mg/kg] favors gut microflora and microflora associated characteristics (Macs) and can prevent dextran sodium sulfate (Dss) induced ulcerative coli- tis in mice model. Dss challenged adult male c57Bl/6 mice were used as model for colon inflammation evaluation. Food and water consumption, disease activity index (Dai), histopathological changes, cytokines in sera and colonic tissues, microflora associated character- istics (Macs; post-fermentation metabolites, short-chain fatty acids, etc.) and gut microflora profiles were studied. We found that GG-β- MOs successfully attenuated colonic inflammation, reduced inflamma- tory proteins, improved the ratio of Firmicutes to Bacteroidetes and promoted the colonization of probiotics like Lactobacillus and Bifidobacteria. Furthermore, GG-β-MOs enriched beneficial bacteria viz., Lactobacillus, Bifidobacteria, Bacillus and Enterococcus showed pos- itive correlation with anti-inflammatory metabolites like D-mannose, coprostanol, pentadecane, alpha-linolenic acid, dihomo-γ-linolenic acid, D-ribose, l-fucose, D-arabinose, N-acetyl-D-glucosamine, acetate and butyrate and negative correlation with oxalate, palmitic acid, ara- chidonic acid and erythritol. Overall, GG-β-MOs supplementation restored altered gut microflora and metabolome leading to improved mucosal repair and reduced intestinal inflammation in Dss induced ulcerative colitis mice model.

Item Type:	Article
Uncontrolled Keywords:	Ulcerative colitis; guar gum β-manno-oligosaccharides; gut microflora; anti-inflammatory; metabolome
Subjects:	600 Technology > 08 Food technology > 10 Food Microorganisms 600 Technology > 08 Food technology > 16 Nutritive value > 01 Carbohydrates
Divisions:	Fermentation Technology and Bioengineering Food Microbiology Molecular Nutrition
Depositing User:	Somashekar K S
Date Deposited:	25 Sep 2025 09:38
Last Modified:	25 Sep 2025 09:38
URI:	http://ir.cftri.res.in/id/eprint/19875

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