Ferulic Acid from Beta vulgaris subsp. vulgaris Altissima Group Pulp Targets the CaMKKβ/SIRT-1/AMPK Pathway To Combat Hyperglycemia

Sangeetha Singh, S. B. and Sachin Eligar, M. and Neelakanteshwar Patil, K. (2025) Ferulic Acid from Beta vulgaris subsp. vulgaris Altissima Group Pulp Targets the CaMKKβ/SIRT-1/AMPK Pathway To Combat Hyperglycemia. Journal of Agricultural and Food Chemistry, 73. pp. 22347-22361.

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Abstract

Hyperglycemia exacerbates type 2 diabetes (T2D), and metformin regulates glucose metabolism via the AMPK signaling pathway. Ferulic acid, a natural antioxidant from Beta vulgaris subsp. vulgaris Altissima Group (sugar beet) pulp (SBP), an agri-processing byproduct, has unclear antidiabetic mechanisms. Our previous study showed that SBP extract improves obesity- induced T2D via the SIRT-1/AMPK pathway in C57BL/6J mice. In this study, we purified ferulic acid from SBP (FA-SBPE) and evaluated its effects on hyperglycemia-induced HepG2 cells. FA-SBPE activated AMPKα/β via its upstream kinase CaMKKβ, upregulated GLUT2 through the SIRT-1/AMPK axis, and modulated the PI3K/Akt pathway by regulating INSR, PIP4K2A, and B gene expression. It enhanced insulin sensitivity, mitochondrial function, and lipid metabolism via PGC-1α and PPARα independently of AMPK. FA-SBPE also inhibited gluconeogenesis, GSK3β, and mTOR signaling. These findings suggest that FA- SBPE modulates glucose metabolism through CaMKKβ/SIRT-1/AMPK and PI3K/Akt/mTOR/GSK3β signaling pathways, making it a potential therapeutic agent for managing hyperglycemia in T2D.

Item Type:	Article
Uncontrolled Keywords:	ferulic acid, sugar beet pulp, AMPK, hyperglycemia, gluconeogenesis, type-2 diabetes
Subjects:	600 Technology > 01 Medical sciences > 04 Diabetes Mellitus 600 Technology > 08 Food technology > 23 Vegetables > 06 Beet Root
Divisions:	Food Microbiology
Depositing User:	Somashekar K S
Date Deposited:	21 Nov 2025 06:34
Last Modified:	21 Nov 2025 06:34
URI:	http://ir.cftri.res.in/id/eprint/20098

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