Modulation of obesity associated metabolic dysfunction by novel lipophilic fraction obtained from Agaricus bisporus

Moumita, Das and Geetha, V and Mehrdad, Zarei and Nanishankar, V. Harohally and Suresh Kumar, G. (2022) Modulation of obesity associated metabolic dysfunction by novel lipophilic fraction obtained from Agaricus bisporus. Life Sciences, 305. pp. 1-12.

PDF 1-s2.0-S0024320522004799-main.pdf - Published Version Restricted to Registered users only Download (2MB) | Request a copy

Abstract

Aim: The present study aimed to prepare a novel lipophilic fraction rich in fat soluble bioactive from Agaricus bisporus and investigated its impact through in vitro and in vivo assessments since the prospective biological activities of fat soluble components from mushrooms are limited. Methods: Ergosterol concentrate fraction (ECF) preparation followed by subsequent characterization of the extract using various analytical techniques (HPLC-UV, Mass spectroscopy, NMR). Furthermore, the fraction has been evaluated for antioxidant activity, DNA protection ability, hypolipidemic properties by in vitro specific enzyme inhibition and in vivo animal model (C57BL/6). Key findings: The fraction majorly contains ergosterol (504 mg/100 g dw) and linoleic acid (71.92 %). In vitro studies showed that the fraction limited free radicals induced DNA damage, exhibited significant free radical scavenging activities (IC50 of DPPH 15.64; ABTS 8.28 mg/ml), and inhibited HMG-CoA reductase activity (IC50 5.03 mg/ml). Further, in vivo study showed that ECF treatment significantly (p < 0.05) improved insulin sensitivity (reduced plasma glucose & insulin, increased adiponectin) and reduced inflammatory markers (CRP & TNF-α) in comparison to high fat fed mice. Furthermore, ECF has significantly reduced plasma lipid profile and accumulation of lipids in liver. This could be due to down regulation of mRNA expression of lipogenic tran- scription factors such as SREBP-1c and SREBP-2, and key lipogenic enzyme ACC. Moreover, ECF treatment has suppressed protein expression of FAS, induced cholesterol clearance by enhancing LDL-R protein expression. Significance: The present work for the first time evaluated the synergistic potential of ergosterol and linoleic acid to improve antioxidant defense system and ameliorate obesity associated metabolic dysfunction.

Item Type:	Article
Uncontrolled Keywords:	White button mushrooms, Antioxidant activities, Ergosterol, Linoleic acid, Obesity-associated metabolic dysfunction
Subjects:	600 Technology > 08 Food technology > 16 Nutritive value > 10 Antioxidant Property 600 Technology > 08 Food technology > 29 Microbiological food > 03 Mushroom
Divisions:	Dept. of Biochemistry Plantation Products Spices and Flavour Technology
Depositing User:	Somashekar K S
Date Deposited:	19 Dec 2025 04:52
Last Modified:	19 Dec 2025 04:52
URI:	http://ir.cftri.res.in/id/eprint/20198

Actions (login required)

View Item