Optimization of isoamyl acetate production by using immobilized lipase from Mucor miehei by response surface methodology.

Hari Krishna, S. and Manohar, B. and Divakar, S. and Prapulla, S. G. and Karanth, N. G. (2000) Optimization of isoamyl acetate production by using immobilized lipase from Mucor miehei by response surface methodology. Enzyme and microbial technology, 26 (2-4). pp. 131-136. ISSN 0141-0229

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Abstract

Immobilized lipase from Mucor miehei was employed for the esterification of isoamyl alcohol with acetic acid in n-heptane solvent. The important process variables studied were enzyme/substrate (E/S) ratio, alcohol (acid) concentration, and incubation period. Based on Box-Behnken design of experiments, a second order response function was developed. The percentage esterification increased with both E/S ratio and time and decreased with alcohol (acid) concentration. The model indicated optimum conditions for maximum esterification ranging from 20 to 99.6% in the alcohol (acid) concentration range of 0.031 to 0.3 M for a range of E/S ratios 8.33 to 50 g/mol, which were in good agreement with the experimental yields.

Item Type:	Article
Uncontrolled Keywords:	Isoamyl acetate; Response surface methodology; Esterification; Lipase; Lipozyme IM; Enzymatic synthesis; Mucor miehei
Subjects:	600 Technology > 08 Food technology > 16 Nutritive value > 05 Enzymes 600 Technology > 08 Food technology > 15 Flavour/Fragrance/Perfumes
Divisions:	Fermentation Technology and Bioengineering Food Engineering
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	26 Nov 2008 11:22
Last Modified:	25 Sep 2018 09:02
URI:	http://ir.cftri.res.in/id/eprint/2174

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