Alteration of superhelical state of DNA by aluminium (Al).

Rao, K. S. and Rao, B. S. and Vishnuvardhan, D. and Prasad, K. V. (1993) Alteration of superhelical state of DNA by aluminium (Al). Biochimica et Biophysica Acta, 1172 (1-2). pp. 17-20. ISSN 0006-3002

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Abstract

The effect of aluminium (Al) on the supercoiled state of pUC18 DNA was studied by ethidium bromide fluorescence and agarose gel electrophoresis. Al at physiologically relevant concentrations relaxed the intact supercoiled DNA as well as the topoisomers induced by chloroquine. EDTA prevented the unwinding effect of Al on supercoiled DNA. Al did not alter the mobility of linear DNA in agarose gels. The implications of this finding in neurological disorders are discussed.

Item Type:	Article
Uncontrolled Keywords:	DNA supercoiling, aluminium
Subjects:	500 Natural Sciences and Mathematics > 07 Life Sciences > 03 Biochemistry & Molecular Biology
Divisions:	Food Safety Analytical Quality Control Lab Dept. of Biochemistry
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	09 Mar 2011 09:46
Last Modified:	28 Dec 2011 09:39
URI:	http://ir.cftri.res.in/id/eprint/2292

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