Aggregation, dissociation and denaturation of sesame (Sesamum indicum L)L-globulin in cetyl trimethyl ammonium bromide solution.

Lakshmi, T. S. and Nandi, P. K. (1977) Aggregation, dissociation and denaturation of sesame (Sesamum indicum L)L-globulin in cetyl trimethyl ammonium bromide solution. International Journal of Peptide and Protein Research, 10. pp. 120-128.

PDF International Journal of Peptide and Protein Research, Volume-10( (1977) 120-128.pdf - Published Version Restricted to Registered users only Download (280kB)

Abstract

The behaviour of the major protein of sesame seed (Sesamum indicum L.) alpha-globulin has been studied in a cationic detergent, cetyl trimethyl ammonium bromide solution. Up to a critical detergent concentration the protein is precipitated from solution, above which redissolution of the protein is observed. Sedimentation velocity patterns indicate the presence of higher aggregates in the detergent concentration range 5 x 10-5 -1 X 10-3 M. These are considered to be the soluble precursors of the insoluble aggregates. Fluorescence measurements show that tryptophanyl groups of the protein which are in contact with the aqueous phase are perturbed by the detergent. The difference spectra of the protein in higher concentration of detergent indicate considerable red shift in the spectrum. Spectrophotometric titration of phenolic groups in 1 X 10-2 M eTAB indicate that a conformational change in the protein has taken place.

Item Type:	Article
Uncontrolled Keywords:	protein sesame seed alpha globulin
Subjects:	500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 29 Protein Chemistry 600 Technology > 08 Food technology > 19 Lipids-oils/fats > 01 Oilseeds
Divisions:	Protein Chemistry and Technology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	28 Dec 2016 07:47
Last Modified:	28 Dec 2016 07:47
URI:	http://ir.cftri.res.in/id/eprint/2762

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