Ascorbic acid-induced loss of a pediocin-encoding plasmid in Pediococcus acidilactici CFR K7.

Ramesh, A. and Halami, P. M. and Chandrashekar, A. (2000) Ascorbic acid-induced loss of a pediocin-encoding plasmid in Pediococcus acidilactici CFR K7. World Journal of Microbiology and Biotechnology, 16 (7). 695-697, 14 ref..

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Abstract

A strain of Pediococcus acidilactici CFR K7 grown in presence of 1 mM ascorbate for 18 h resulted in 35% colony-forming units (CFU) irreversibly losing their ability to produce pediocin. Agarose gel electrophoresis of plasmid DNA and dot-blot hybridization showed concomitant loss of a 7.8 kb plasmid coding for pediocin from these colonies. Sensitivity of these colonies to nitrofurantoin was also observed after ascorbate treatment. Since ascorbic acid is a readily available and non-hazardous compound in contrast to other conventional plasmid curing agents, the possibility of its use in determining plasmid-encoded traits in food grade lactic acid bacteria is also proposed.

Item Type:	Article
Uncontrolled Keywords:	Ascorbic acid, pediocin, plasmid curing
Subjects:	500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	06 Jun 2011 11:39
Last Modified:	28 Dec 2011 09:42
URI:	http://ir.cftri.res.in/id/eprint/3113

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