Biosynthesis of choline and its incorporation by resting cells of Neurospora crassa.

Dalal, F. R. and Rege, D. V. and Sreenivasan, A. (1961) Biosynthesis of choline and its incorporation by resting cells of Neurospora crassa. Journal of Scientific and Industrial Research, 20C. pp. 255-258.

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Abstract

Attempts have been made to demonstrate the synthesis of choline by respiring mycelia of N. crassa (wild) from ethanolamine and various methyl precursors under varied experimental conditions. In no case a net increase in choline content has been observed. The abUity of the cells to bring about the synthesi.s could be inferred from the presence of this metabolite in the mycelium harvested from the chollneless medium. More directly the ability to incorporate C14 from (C14Ha) methionine or (3-C14) serine has been demonstrated. Mycelia of both the wild type and the cholineless strai.ns can rapidly incorporate choline into the cells, the amount incorporated increasing with the concentration of choline in the suspension medium up to 50 ug./ml. A balance sheet reveals that during the 4 hr period of incubation much (up to 30 per cent) of the choline was lost in unaccountable ways. This is not due to oxidation, demethylation, conversion to trimethylamine or degradations leading to liberation of free ammonia.

Item Type:	Article
Uncontrolled Keywords:	synthesis of choline, mycelia of N. crassa
Subjects:	500 Natural Sciences and Mathematics > 07 Life Sciences > 03 Biochemistry & Molecular Biology > 04 Biosynthesis
Divisions:	Dept. of Biochemistry
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	22 Jul 2016 05:56
Last Modified:	22 Jul 2016 05:56
URI:	http://ir.cftri.res.in/id/eprint/3193

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