Development of media for growth of Dioscorea deltoidea cells in in vitro diosgenin production: Influence of media constituents and nutrient stress.

Ravishankar, G. A. and Grewal, C. (1991) Development of media for growth of Dioscorea deltoidea cells in in vitro diosgenin production: Influence of media constituents and nutrient stress. Biotechnology Letters, 13 (2). pp. 125-130.

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Abstract

Callus culture of Dioscorea deltoidea produced diosgenin and sterols during stationary phase. Ammonium nitrate (420 mg Nitrogen/l) as sole nitrogen source supported better growth than a combination of ammonium nitrate and potassium nitrate (totally equivalent to 840 mg Nitrogen/l). The production of diosgenin increased under low phosphate concentration (100 mg/l) whereas high phosphate concentration (240 mg/!) promoted growth. Micronutrients, when used at 1ı89 strength, enhanced growth and diosgenin production. Depletion of nitrogen increase in the diosgenin synthesis by a factor of 2. Adoption of a two stage culture method enhanced the diosgenin production in cultured cells by eight-fold.

Item Type:	Article
Uncontrolled Keywords:	Callus culture, Dioscorea deltoidea, diosgenin, sterols, plant ceils culture
Subjects:	500 Natural Sciences and Mathematics > 10 Plants 500 Natural Sciences and Mathematics > 10 Plants > 05 Tissue Culture
Divisions:	Plant Cell Biotechnology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	14 Mar 2018 05:22
Last Modified:	14 Mar 2018 05:22
URI:	http://ir.cftri.res.in/id/eprint/3769

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