Spectrophotometric assay of immobilized tannase.

Katwa, L. C. and Ramakrishna, M. and Raghavendra Rao, M. R. (1981) Spectrophotometric assay of immobilized tannase. Journal of Biosciences, 3. pp. 135-142.

PDF J. Biosci., Vol. 3 Number 2, June 1981, pp. 135-142.pdf - Published Version Restricted to Registered users only Download (315kB) | Request a copy

Abstract

A procedure for the assay of immobilized tannase with Polyacrylamide gel, collagen and Duolite-S-762 as matrices is described. It is based on the spectrophotometric determination of gallic acid formed by the enzymatic hydrolysis of tannic acid. The kinetic parameters of the enzymatic reaction have been studied and an assay procedure has been formulated. This method appears to be much more accurate than those reported earlier.

Item Type:	Article
Uncontrolled Keywords:	Tannase; immobilization; gallic acid; tannic acid; spectrophotometric assay
Subjects:	500 Natural Sciences and Mathematics > 07 Life Sciences > 03 Biochemistry & Molecular Biology > 07 Enzyme Biochemistry
Divisions:	Dept. of Biochemistry
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	10 Apr 2018 06:51
Last Modified:	10 Apr 2018 06:51
URI:	http://ir.cftri.res.in/id/eprint/5455

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