Site-directed antibody immobilization on gold substrate for surface plasmon resonance sensors.

Hirlekar Schmid, A. and Stanca, S. E. and Thakur, M. S. and Ravindranathan Thampia, K. and Raman Suri, C. (2006) Site-directed antibody immobilization on gold substrate for surface plasmon resonance sensors. Sensors and Actuators B, 113 (1). pp. 297-303. ISSN 0925-4005

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Abstract

A simple method for the site-directed immobilization of antibodies on gold substrates has been developed for surface plasmon resonance (SPR) applications. A freshly prepared gold surface on a glass slide was modified with Protein A via homobifunctional cross linker dithiobissuccinimide propionate (DSP) to achieve uniform, stable, and sterically accessible antibody coating. The density of the immobilized antibodies bound to Protein A was examined as a function of the protein concentration, buffer pH, buffer type and reaction time. The suggested scheme was also employed for the modification of gold-coated substrates for quartz crystal microbalance (QCM) applications. The modified gold surface was stable for several weeks and the reproducibility was satisfactory. The current fabrication technique could be applied to construct other immunosensors or biochips.

Item Type:	Article
Additional Information:	Copyright of this article belongs to Elsevier Ltd.
Uncontrolled Keywords:	Protein A; Dithiobis-succinimidyl propionate; Immobilization; SPR
Subjects:	600 Technology > 05 Chemical engineering > 01 Biotechnology and Bioengineering
Divisions:	Fermentation Technology and Bioengineering
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	07 Apr 2007
Last Modified:	28 Dec 2011 09:27
URI:	http://ir.cftri.res.in/id/eprint/570

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