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The calculation of partial specific volumes of proteins in 8M urea solution.
Prakash, V. and Timasheff, S. N. (1981) The calculation of partial specific volumes of proteins in 8M urea solution. Analytical Biochemistry, 117. pp. 330-335.
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Analytical_Biochemistry,_Volume_117,_Issue_2,_1_November_1981,_Pages_330-335.pdf Restricted to Registered users only Download (484kB) |
Abstract
A method for calculating the number of urea and water molecules bound to proteins in 8 M urea solution in acidic pH is presented. The preferential interaction parameter of solvent components with proteins can be obtained by a proper combination of the calculated number of urea and water molecules bound to the protein. This in turn can give accurate values of the apparent partial specific volumes of proteins in 8 M urea solution. The method demands only a knowledge of the amino acid composition of the protein.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | water molecules, proteins, amino acid composition |
| Subjects: | 500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 29 Protein Chemistry |
| Divisions: | Protein Chemistry and Technology |
| Depositing User: | Food Sci. & Technol. Information Services |
| Date Deposited: | 01 Jun 2011 09:41 |
| Last Modified: | 28 Dec 2011 09:51 |
| URI: | http://ir.cftri.res.in/id/eprint/5941 |
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