Carbohydrates and their degrading enzymes from native and malted finger millet (Ragi, Eleusine coracana, Indaf-15)

Nirmala, M. and Subba Rao, M. V. S. S. T. and Muralikrishna, G. (2000) Carbohydrates and their degrading enzymes from native and malted finger millet (Ragi, Eleusine coracana, Indaf-15). Food Chemistry, 69. pp. 175-180. ISSN 0308-8146

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Abstract

A recently released hybrid ragi, Indaf-15 was germinated up to 96 h at 25C and the sprouts, drawn at 24 h intervals, were dried,devegetated, powdered and evaluated for malting loss, reducing sugar, free sugar profile, starch content, dietary fibre and an array of carbohydrate-degrading enzymes. Malting loss was maximum (32.5%) at 96 h. The total reducing sugar content increased from 1.44 to 8.36%, whereas the total carbohydrate content decreased from 81 to 58% at 96 h of germination. Analysis of 70% alcohol- soluble sugars revealed glucose, fructose and sucrose in different proportions with respect to germination time. Maltose and mal-totriose were detected after 48 and 72 h, respectively. There was a linear decrease in starch content (from 65 to 43%). Activities of amylase and pullulanase were maximum at 72 h whereas those of α-D-glucosidase and 1,3-β-d-glucanase, were maximum at 48 h. Xylanase activity was maximum at 96 h with a concomitant decrease in arabinose to xylose ratio from 1:1 to 1:0.38 in the dietary fibre. α-Galactosidase activity was negligible, which is in tune with a very small amount of raffinose series oligosaccharides. The above results indicated that Indaf-15 is a potential variety for malting purposes as it develops high levels of amylases during germination, and its malt form is a rich source of reducing sugar.

Item Type:	Article
Subjects:	600 Technology > 08 Food technology > 16 Nutritive value > 01 Carbohydrates
Divisions:	Dept. of Biochemistry
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	06 Jun 2005
Last Modified:	17 Oct 2018 09:28
URI:	http://ir.cftri.res.in/id/eprint/7

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