Fructooligosaccharide production using fructosyl transferase obtained from recycling culture of Aspergillus oryzae CFR 202.

Sangeetha, P. T. and Ramesh, M. N. and Prapulla, S. G. (2005) Fructooligosaccharide production using fructosyl transferase obtained from recycling culture of Aspergillus oryzae CFR 202. Process Biochemistry, 40 (3-4). pp. 1085-1088.

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Abstract

Fructooligosaccharide (FOS) production was carried out using fructosyl transferase (FTase) produced by Aspergillus oryzae CFR 202 under submerged fermentation conditions. The pellets of A. oryzae CFR 202 obtained after 48 h of fermentation were supplemented with fresh media after every 24 h and fermentation was carried out to produce FTase. FTase so obtained was used to produce FOS using 60% sucrose as substrate at 55 ◦C at pH 5.15. FTase activity was maintained in the range of 15 ± 2 U/ml/min up to six recycles. FOS yields were maintained at 53% up to 6th cycle. Recycling of pellets could not be carried out after 6th cycle due to disintegration. The system is advantageous and economical in that it does not require supplementation of any additional nutrients nor it requires the development of fresh inoculum.

Item Type:	Article
Uncontrolled Keywords:	ASPERGILLUS-; BIOMASS-; FERMENTATION-TECHNOLOGY; GLYCOSYLTRANSFERASES-; POLYSACCHARIDES-; SUGARS-; ASPERGILLUS-ORYZAE; FRUCTOOLIGOSACCHARIDES-; FRUCTOSYLTRANSFERASES-
Subjects:	600 Technology > 08 Food technology > 25 Sugar/Starch/Confectionery
Divisions:	Fermentation Technology and Bioengineering
Depositing User:	Users 197 not found.
Date Deposited:	02 Jun 2011 09:52
Last Modified:	28 Dec 2011 09:58
URI:	http://ir.cftri.res.in/id/eprint/7528

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