Response surface methodology for optimization of growth parameters for the production of carotenoids by a mutant strain of Rhodotorula gracilis.

Vijayalakshmi, G. and Shobha, B. and Vanajakshi, V. and Divakar, S. and Manohar, B. (2001) Response surface methodology for optimization of growth parameters for the production of carotenoids by a mutant strain of Rhodotorula gracilis. European Food Research and Technology, 213 (3). 234-239, 8 ref..

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Abstract

Response surface methodology of growth parameters for carotenoid synthesis by a mutant strain of Rhodotorula gracilis (CFR 0-1) was performed by a fractional factorial design involving 5 variables: 4-8% glucose; 6-12 days incubation; inoculum vol. 6-12 ml/100 ml; pH 4.5-7.7; and temp. 24-32'b0C. The response equation developed indicated a linear relationship between period of incubation, temp., sugar concn. and vol. of inoculum for carotenoid production. Results indicated max. carotenogenesis (0.09%) at 8% glucose level, pH 7.5 and 6.0 ml/100 ml of inoculum for an incubation period of 12 days at 24'b0C. These theoretical values were verified by experimental data.

Item Type:	Article
Uncontrolled Keywords:	BACTERIA-; CAROTENOIDS-; FERMENTATION-TECHNOLOGY; RHODOTORULA-GRACILIS
Subjects:	500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology
Divisions:	Food Microbiology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	11 Feb 2011 09:45
Last Modified:	16 May 2012 07:11
URI:	http://ir.cftri.res.in/id/eprint/7630

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