Genesis and Development of DPPH Method of antioxidant determination

Sagar, B. Kedare (2008) Genesis and Development of DPPH Method of antioxidant determination. [Student Project Report]

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Abstract

This Dissertation / Report is the outcome of investigation carried out by the creator(s) / author(s) at the department/division of Central Food Technological Research Institute (CFTRI), Mysore mentioned below in this page.

Item Type:	Student Project Report
Additional Information:	The antioxidant network, due to its wide range of redox potential, localization and degree of activity allows complete and efficient protection against free radical attack generated in the different cellular environments. The efficiency of the antioxidant network is related to free radical production, potency and concentration of antioxidants, gene expression and factors still unknown. There are various methods for determination of antioxidant potential of different commodities. However, one method is not suitable for all. There is no shortcut approach to determine antioxidant activity. DPPH method offers advantages of being rapid, simple and inexpensive method and provides first hand information on the overall antioxidant capacity. However, the absorbance of DPPH is decreased by light exposure, oxygen content, pH, and solvent types. Quantitative data of antioxidant ability of compound can be obtained when these factors are considered . The trend in antioxidant activity obtained by using the DPPH method is comparable to trends found using other methods reported in the literature. The DPPH method has been widely applied for estimating antioxidant activity in recent years, but its applications should to be carried out bearing in mind the basis of the method, and the need wherever possible to establish the stoichiometry for the quenching reaction, so that the antioxidant activity may be related to the structure of the substrate molecule. For a better understanding of the mechanisms involving the DPPH radical and potential antioxidants, it would be interesting to characterize the reaction intermediates and products. To do this, it is necessary to separate these compounds by chromatography and to identify them. It would also be very useful to build a plausible kinetic model and determine the order of the different reactions and their constants. Thus, important improvements in DPPH method were discussed, which gives a wide range of applications based on the requirement and type of affordable inputs.
Uncontrolled Keywords:	DPPH method antioxidant assay
Subjects:	600 Technology > 08 Food technology > 32 Antioxidants
Divisions:	Human Resource Development
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	16 Mar 2009 06:45
Last Modified:	01 Jul 2015 11:09
URI:	http://ir.cftri.res.in/id/eprint/8916

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