Monoclonal antibodies that block cell adhesion in Polysphondylium pallidum: reaction with L-fucose, a terminal sugar in cell-surface glycoproteins

Katsumi, Toda and Rudrapatham, N. Tharanathan (1984) Monoclonal antibodies that block cell adhesion in Polysphondylium pallidum: reaction with L-fucose, a terminal sugar in cell-surface glycoproteins. European Journal of Biochemistry, 143 (3). pp. 477-481.

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Abstract

By permethylation analysis the linkages of L-fucose and D-mannose in the oligosaccharide residues of cell surface glycoproteins of Polysphondylium pallidum were determined. Mannose was found in terminal positions, in 1,2-, 1,3- and 1,6-intra-chain linkages, and at branch points. Fucose was exclusively located at nonreducing ends. Fab of a monoclonal antibody, mAb 293, has been previously shown to inhibit cell adhesion in P. pallidum completely. Binding of this antibody to glycoprotein was blocked by L-fucose, and at very high concentrations also by D-mannose. The dissociation constant for the antibody-fucose complex was Kd= 70μM, which was two orders of magnitude higher than estimated for the natural oligosaccharide. Antibody-glycoprotein complexes dissociated in the presence of 100 mM L-fucose with a half-time of about 56s. The blockage by L-fucose is taken as evidence that the adhesion-blocking antibody binds to oligosaccharide end groups containing L-fucose as the terminal sugar.

Item Type:	Article
Uncontrolled Keywords:	L-fucose, Polysphondylium pallidum, glycoproteins
Subjects:	500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 28 Polysaccharide Chemistry
Divisions:	Dept. of Biochemistry
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	16 Sep 2010 11:36
Last Modified:	28 Dec 2011 10:18
URI:	http://ir.cftri.res.in/id/eprint/9684

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