Reaction Kinetics of the Invertase from Yeast (S. Cerevisiae).

Invertase converts sucrose to glucose and fructose.
The reaction mechanism for the formation of glucose and
fructose was studied by stopped flow spectrophotometer and
circular dichroism. The reaction mechanism follows biphasic
mode with rate constants of k10.0053 s−1±0.001 s−1 and k2
0.030 s−1±0.01 s−1 for 25 mM concentration of sucrose. Far
UV circular dichroic spectrum of invertase in presence of
sucrose shows 18 % increase in β conformation as a function
of time. Taken together, the invertase hydrolysis follows biphasic
mode where it undergoes conformational changes
followed by hydrolysis of the sucrose.