Diffusion, Frowning and Smiling of Low Molecular Weight Protein Bands: A Simple, Rapid and Efficient Solution

A modified method of SDS-polyacrylamide gel
electrophoresis for the efficient separation of low molecular
weight proteins (ranging 1.4–10 kDa, containing disulfide
bonds) without frowning, smiling and diffusion is
described. SDS-polyacrylamide and tricine gel electrophoresis
are considered as standard approaches for low
molecular weight protein separation, despite frowning,
smiling and diffusion problems. These methods are inefficient
and confusing as Coomassie brilliant blue stained low
molecular weight protein bands are difficult to identify
since proteins get diffused and, it is difficult to identify as
proteins. The method developed offers a solution to the
existing problem and is more suitable for the efficient
separation of low molecular weight proteins with high
resolution (with di-sulfide bonds).