Hydrolysis of D,L-tryptophan isopropyl este catalysed by Beta-cyclodextrin.

ß-Cyclodextrin (BCD) catalysed hydrolysis of D,Ltryptophan
isopropyl ester is found to be pH dependent.
The pH rate constant profiles show that the kobs
values for the BCD catalysed reactions are higher than
those of the control in the pH range 9.8 to 12.9. The
reaction follows Michaelis-Menten type kinetics exhibiting
substrate saturation. Michaelis-Menten treatment results
in determination of KD (0.067 M) and kcat (0.015
M-1 min-1). A kcat/kun values of 0.94 has been determined.
Tryptophan, the hydrolysis product formed exhibited
enantiometric excess of 7.9 to 18.4% of the ( -)
antipode in reactions catalysed by BCD, heptakis-2,6-
di-O-methyl-ß-cyclodextrin (DM-BCD), BCD-epichlorohydrin
polymer (BCD-polymer) and a 1:1 complex of
Cu(II)-ß-cyclodextrin(Cu(II)-BCD).