Galectin-3 in urine of cancer patients: stage and tissue specificity

Kiruthika, Balasubramanian and Raghavendra, Vasudevamurthy and Sathisha, U. V. and Anil, Thomas (2009) Galectin-3 in urine of cancer patients: stage and tissue specificity. Journal of Cancer Research and Clinical Oncology, 135. pp. 355-363.

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Abstract

Purpose Galectin-3 has been implicated in advanced stage of cancer disease. In the current study we examined the possibility of urinary galectin-3 levels to stage cancer disease and to follow up therapy. Experimental design Urine was collected from all types of cancer patients at diVerent stages including patients undergoing radio/chemotherapy. Galectin-3 level was determined by anti-galectin-3 based ELISA and agglutination assays. Immunoblotting and puriWcation on lactosyl aYnity column further conWrmed the presence of galectin-3. Results Cancer samples exhibited stage dependent expression of galectin-3 approx. ranging from 1.0 to 3.3, 4.4 to 5.4, 5.4 to 24.7, 13.1 to 31.9, 13.9 to 32.9 ng/mg C (creatinine) for stage I–V, respectively, at P » <0.05 level. Galectin-3 levels were decreased by approx. threefolds after 5th day of therapy. Conclusions Sample collection being simple and noninvasive, urinary galectin-3 may be used as a potential diagnostic tool for monitoring or follow up of the stage of cancer disease.

Item Type:	Article
Uncontrolled Keywords:	Galectin-3 · Hemagglutination · Creatinine · Metastasis · Radio/chemotherapy · Urine
Subjects:	500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 29 Protein Chemistry 600 Technology > 01 Medical sciences
Divisions:	Dept. of Biochemistry
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	07 Jun 2011 06:53
Last Modified:	29 Oct 2018 04:06
URI:	http://ir.cftri.res.in/id/eprint/10080

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